Mixed models have become important in analyzing the results of experiments, particularly those that require more complicated models such as those that involve longitudinal data. A method for deriving the terms in a mixed model, described by Brien and Demétrio (2009) [J. Agric. Biol. and Env. Stat., 14, 253-80] will be presented. It extends the method described by Brien and Bailey [J. Roy. Stat. Soc., Series B, 68 (2006): 571-609] to explicitly identify terms for which autocorrelation and smooth trend, arising from longitudinal observations, needs to be incorporated in the model. At the same time we retain the principle that the model used should include, at least, all the terms that are justified by the randomization. This is done by dividing the factors into sets, called tiers, based on the randomization and determining the nesting and crossing relationships between factors.To illustrate the method, a mixed model for the randomized complete block design with longitudinal observations is outlined. The mixed model analysis of data from a three-phase experiment to investigate the effect of time of refinement on Eucalyptus pulp from four different sources is also described. For this example, cubic smoothing splines are used to describe differences in the trend over time and unstructured covariance matrices between times are found to be justified.

Keywords: Mixed models; Longitudinal experiment; Repeated Measures; Agricultural experiments

Biography: I am Professor in Experimental Statistics at Escola Superior de Agricultura “Luiz de Queiroz”, University of São Paulo, Head of Exact Sciences Department. I have a Degree in Agonomy, Master and PhD in Applied Statistics to Agriculture and Post-Doctoral at Matemathics Department, Imperial College of Science and Technology, Londres, Inglaterra. I teach for undergraduate and graduate courses and directs a research program dealing with Statistics in Agriculture. I have published topics including generalized linear models and extensions and mixed models applied to Agriculture.